Biotechnology is applied to the biological production of hydrogen.

Implementation period

December 2021 to August 2022

Scope of work

Digital technology

Resilient infrastructure







This project has been funded by the national programme for clusters support “Ayudas a Agrupaciones Empresariales Innovadoras” (AEIs) of the Ministry of Industry, Commerce and Tourism. Recovery, Transformation and Resilience Plan – Funded by the European Union – NextGenerationEU.


BIO-H2 proposes a green biological H2 generation system using wastewater organic matter as a substrate. This system is planned as an economical and modular solution that allows the valorisation of wastewater produced in the industry, mainly if it contains a high concentration of sugars in its effluents, as is the case in the agri-food industry.

We propose three lines of work to improve bacterial cultures, optimise hydrogen production processes, and store and control hydrogen.

BIO-H2 will sensor all system’s critical parameters, providing real-time monitoring of the entire process. The second part of this project will focus on the energetic use of the hydrogen produced for self-consumption.


The objective of BIO-H2 is the biological production of green hydrogen using wastewater as feedstock. This green hydrogen, used as energy self-consumption in an industry, will decrease the company’s energy costs and reduce its carbon footprint. In addition, the biological process will increase the biodegradability of the water, thus reducing wastewater treatment costs.

On the other hand, BIO-H2 will produce a waste valorisation and a complete circular economy with almost zero energy generation cost if we consider reducing water treatment costs.

The whole system is based on the idea that it should operate autonomously and with minimum maintenance. Therefore, the entire hydrogen generation, water treatment and gas storage system will be digitalised and sensorised in real-time with 4.0 technology to work harmoniously. All this information will be integrated into a control platform that will send automatic alerts when variables go out of the established range.


We can define an improved metabolic route for increasing H2 production by adjusting the main influencing variables. In addition, the design of the gas purification and storage system will be obtained. All of this, under the principles of Industry 4.0